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Graham Calder |
Facilities
support research in:
• Protein Metabolism
• Structural Analysis
• Quantitative Analysis by Isotope Dilution |
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The section has 4 gas chromatography
mass spectrometers (GC/MS). These instruments are mainly used to
quantitate compounds of biological interest by isotope dilution
and to measure the enrichment in deuterium, carbon-13 or nitrogen-15
in target metabolites in relation to kinetic studies using stable
isotopes as tracers. These metabolites are isolated from biological
fluids using chromatography techniques prior to derivatisation and
GC/MS analysis. The instruments have the ability to operate in two
modes of ionisation. Chemical ionisation (CI) and electron impact
ionisation (EI). CI is a soft ionisation technique which is used
to enhance the molecular ion, this is a very sensitive technique
requiring only small quantities (pg) of material. Electron impact
ionisation (EI) requires more material (ng) but looks at unique
fragments derived from each metabolite. Using EI allows the isotopic
labelling in different atoms of the molecule to be defined (isotopomer
analysis) and thus can support more complex metabolic studies utilising
multiple tracers. |
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Instruments available: |
Agilent 5973 MSD |
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• 6890N Gas Chromatograph
• 7683 Auto sampler
• Electron impact (EI)
• Positive and Negative Chemical Ionization (CI)
• Isotopes measured: - 13-C , 15-N, 2-H |
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HP 5989A MS 'Engine' Quadrupole Mass
Spectrometer |
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• HP 5890 Series II Gas
Chromatograph
• HP 7673 Auto sampler
• Electron impact (EI)
• Positive and Negative Chemical Ionization (CI)
• Isotopes measured: - 13-C , 15-N, 2-H
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Thermoquest Trio-1 Quadrupole Mass
Spectrometer |
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• HP 5890 Series II Gas
Chromatograph
• HP 7673 Auto sampler
• Electron impact (EI) Ionization only
• Isotopes measured: - 13-C , 15-N, 2-H |
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Thermoquest 'Voyager' Quadrupole Mass
Spectrometer |
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• GC 8000 Top Gas Chromatograph
• AS 800 Auto sampler
• Electron impact (EI)
• Positive and Negative Chemical Ionization (CI)
• Isotopes measured: - 13-C , 15-N, 2-H |
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• Contact
Mass Spectrometry Section (Analytical Facilities Staff list)
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Contact details for Graham Calder
Email:G.Calder@abdn.ac.uk
Telephone:+44 (0)1224 712751 ext.7225 |
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